Taxonomic identification report

Sample ID: VE25-1434_COI
Reference database: BLAST

Report generated from the Taxonomic Identification Nextflow workflow

This report describes the results of a taxonomic identification analysis for the sample VE25-1434_COI. The analysis compares the sample's DNA sequence against a reference database of sequences with known taxonomy. The results include the best matches from the database, which are referred to as "Candidates". To provide rigour, the report also includes an analysis of supporting publications (a measure of the integrity of candidate hit sequences), and database coverage of the different taxa that are of interest to the report.

Facility	QCIF
Analyst	Magdalena Antczak
Analysis start	`2025-07-01 02:19:05`
Analysis end	`2025-07-01 05:12:47`
Wall time	`2:53:42 hours`

Result overview

Dohrniphora cornuta

Flag 1A

Outcome: Positive species identification.
Reasoning: 1 candidate species matched with high stringency (identity ≥ 98.5%).

*Preliminary morphology ID Phoridae* confirmed?**	True
Flag 7A Outcome: The preliminary identification is supported by the molecular data. Reasoning: Identified species is consistent with preliminary ID.

This Preliminary Morphology ID has been independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of this taxon. Insufficient coverage can result in that taxon not be correctly identified as the taxonomic identity of the sample (type-II error).

For example, if your sample is from Homo sapiens, but there are no Homo sapiens DNA records in the reference database, the analysis will be unable to detect Homo sapiens as the correct taxonomic identity. In the latter case, the analysis will most likely try to assign the closest relative with existing reference data as the taxonomic identity.

Reference database: BLAST Core Nt

	Coverage of Phoridae
	Coverage of species in genus Phoridae
	Coverage of species in genus Phoridae in country of origin India

Database coverage of Phoridae

Flag 5.1NA: This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species

107,626 records

There are 107,626 sequence records in the reference database for Phoridae that have been annotated with the given locus COI. It is possible that more records exist for this taxon which were not correctly annotated with this locus.

Global occurrence records for Phoridae.
Note that the occurrence data are not exhaustive, since the data fetched are limited to 5000 occurrence records. It is therefore possible for this species to occur in regions not shown on the map.

Database coverage of species in genus Phoridae

Flag 5.2NA: This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species

Database coverage of species in genus Phoridae that occur in country of origin India

Flag 5.3NA: This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species

Taxa of interest detected?

False

Flag 2B

Outcome: None of the taxa of interest matched the candidates species.
Reasoning: Taxon of interest NOT detected in candidate species.

Flag 5.1A

Outcome: The reference data supports this taxon well.
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries).

Flag 5.2B

Outcome: The reference data offers some support for species in this genus.
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus.

Sample metadata ⓘ

Sample ID	VE25-1434_COI
Locus	COI
Preliminary ID	Phoridae
Taxa of interest	Megaselia scalaris
Country	India
Host	Fish meal
Query DNA sequence

`db_type`	`blast_core_nt`
`metadata`	`/mnt/data/tests-wf-2//input/blast_core_nt/all_20250529_metadata.csv`
`outdir`	`/mnt/data/tests-wf-2//results/blast_core_nt/all_20250529/v100_20250701`
`sequences`	`/mnt/data/tests-wf-2//input/blast_core_nt/all_20250529_query.fasta`
`bold_skip_orientation`	`0`
`max_candidates_for_analysis`	`3`
`median_identity_warning_factor`	`0.95`
`min_identity`	`0.935`
`min_identity_strict`	`0.985`
`min_nt`	`300`
`min_q_coverage`	`0.85`
`phylogeny_min_hit_identity`	`0.95`
`phylogeny_min_hit_sequences`	`10`
`allowed_loci_file`	`/home/ubuntu/nextflow-pipeline/daff-taxassignwf/scripts/config/loci.json`
`db_cov_country_missing_a`	`1`
`db_cov_min_a`	`5`
`db_cov_min_b`	`1`
`db_cov_related_min_a`	`90`
`db_cov_related_min_b`	`10`
`db_coverage_toi_limit`	`10`
`gbif_accepted_status`	`accepted,doubtful`
`gbif_limit_records`	`500`
`gbif_max_occurrence_records`	`5000`
`min_source_count`	`5`
`blast_database_name_for_report`	`BLAST Core Nt`
`blast_max_target_seqs_for_report`	`2000`
`report_debug`	`0`
`email`	`None`
`logging_debug`	`0`
`accessions_filename`	`accessions.txt`
`blast_xml_filename`	`blast_result.xml`
`hits_fasta_filename`	`all_hits.fasta`
`hits_json_filename`	`all_hits.json`
`boxplot_img_filename`	`candidates_identity_boxplot.png`
`candidates_csv_filename`	`candidates.csv`
`candidates_fasta_filename`	`candidates.fasta`
`candidates_phylogeny_fasta_filename`	`candidates_phylogeny.fasta`
`candidates_json_filename`	`candidates.json`
`candidates_sources_json_filename`	`candidates_sources.json`
`independent_sources_json_filename`	`aggregated_sources.json`
`bold_taxonomy_json`	`bold_taxonomy.json`
`taxonomy_filename`	`taxonomy.csv`
`candidates_msa_filename`	`candidates_phylogeny.msa`
`tree_nwk_filename`	`candidates_phylogeny.nwk`
`email_on_fail`	`None`
`plaintext_email`	`False`
`monochrome_logs`	`False`
`help`	`False`
`help_full`	`False`
`show_hidden`	`False`
`version`	`False`
`pipelines_testdata_base_path`	`https://raw.githubusercontent.com/nf-core/test-datasets/`
`trace_report_suffix`	`2025-07-01_02-19-03`
`config_profile_name`	`None`
`config_profile_description`	`None`
`custom_config_version`	`master`
`blastdb`	`/home/ubuntu/blastdbs/20250329/core_nt`
`taxdb`	`/home/ubuntu/.taxonkit/`
`analyst_name`	`Magdalena Antczak`
`facility_name`	`QCIF`
`ncbi_api_key`	`d2c2e49b459493d627948bc7d5a07fca5c08`
`ncbi_user_email`	`magdalena.antczak@qcif.edu.au`
`custom_config_base`	`https://raw.githubusercontent.com/nf-core/configs/master`
`config_profile_contact`	`None`
`config_profile_url`	`None`
`validate_params`	`True`

Analyst evaluation

Identification of candidate species

Flag 1A

Outcome: Positive species identification.
Reasoning: 1 candidate species matched with high stringency (identity ≥ 98.5%).

ⓘ BLAST hits must meet ONE of these criteria to be considered for candidate screening:

Minimum alignment length ⓘ	`300bp`
Minimum query coverage ⓘ	`85.0%`

ⓘ BLAST hits have been classified as follows:

Classification	Alignment identity ⓘ	Number of hits ⓘ	Number of species ⓘ
STRONG MATCH	≥ 98.5%	1	1
MODERATE MATCH	≥ 93.5%	6	1
NO MATCH	< 93.5%	493	62

Candidate species

Species No. hits ⓘ Top identity ⓘ Median identity ⓘ Top E-value ⓘ Database coverage ⓘ

Dohrniphora cornuta

99.6%

97.8%

0.0

This Candidate has been independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of this taxon. Insufficient coverage can result in that taxon not be correctly identified as the taxonomic identity of the sample (type-II error).

Reference database: BLAST Core Nt

	Coverage of Dohrniphora cornuta
	Coverage of species in genus Dohrniphora
	Coverage of species in genus Dohrniphora in country of origin India

Database coverage of Dohrniphora cornuta

Flag 5.1A: The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)

16 records

There are 16 sequence records in the reference database for Dohrniphora cornuta that have been annotated with the given locus COI. It is possible that more records exist for this taxon which were not correctly annotated with this locus.

Global occurrence records for Dohrniphora cornuta.
Note that the occurrence data are not exhaustive, since the data fetched are limited to 5000 occurrence records. It is therefore possible for this species to occur in regions not shown on the map.

Database coverage of species in genus Dohrniphora

Flag 5.2B: The reference data offers some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus

/ (%) of species have sequence records in the reference database for:

Species in the genus Dohrniphora
At the target locus COI

Number of GenBank records at locus COI

Database coverage of species in genus Dohrniphora that occur in country of origin India

Flag 5.3C: It's unlikely that the true taxonomic identity is another species in this genus, because no others have been recorded in the sample's country of origin
Reasoning: No species in genus have been observed in the country of origin

/ (%) of species have sequence records in the reference database for:

Species in the genus Dohrniphora
At the target locus COI
In the sample country of origin India

Number of GenBank records at locus COI

This table shows all results that were returned by the BLAST search. Each row represents a reference sequence that matched the query sequence. Use CTRL+F to search the table, and click table headers to sort by column. Click on a row to show the corresponding BLAST alignment and taxonomy information in the bottom panes.

Rank ⓘ	Accession ⓘ	Hit subject ⓘ	Align length ⓘ	Query coverage ⓘ	Bitscore ⓘ	E-value ⓘ	Identity ⓘ
1	KR666968	Dohrniphora cornuta voucher BIOUG00942-F05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	535	93.9%	1043.16	0.00e+00	99.6%
2	MG947378	Dohrniphora cornuta voucher 7508_013 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	570	100.0%	1049.11	0.00e+00	97.9%
3	HQ984503	Dohrniphora cornuta voucher BIOUG	570	100.0%	1045.14	0.00e+00	97.9%
4	KR659695	Dohrniphora cornuta voucher BIOUG00938-C02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	570	100.0%	1037.22	0.00e+00	97.8%
5	MG293276	Dohrniphora cornuta voucher BIOUG31051-H09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	560	98.2%	1005.5	0.00e+00	97.4%
6	JQ941749	Dohrniphora cornuta isolate 49 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	535	93.9%	892.51	0.00e+00	96.1%
7	MN832849	Dohrniphora cornuta mitochondrion, complete genome	535	93.9%	892.51	0.00e+00	96.1%
8	OQ532023	Phoridae sp. voucher ZRCBDP0375063 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	536	94.0%	743.841	0.00e+00	92.5%
9	OQ530590	Phoridae sp. voucher ZRCBDP0365007 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	535	93.9%	741.858	0.00e+00	92.5%
10	OQ530389	Phoridae sp. voucher ZRCBDP0364768 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	536	94.0%	735.912	0.00e+00	92.4%
11	OQ530781	Phoridae sp. voucher ZRCBDP0365243 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	536	94.0%	735.912	0.00e+00	92.4%
12	OQ532499	Phoridae sp. voucher ZRCBDP0375585 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	535	93.9%	733.929	0.00e+00	92.3%
13	OQ527298	Phoridae sp. voucher ZRCBDP0360646 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	535	93.9%	733.929	0.00e+00	92.3%
14	MN520900	Dohrniphora sp. SAEVG Morph0080 cytochrome oxidase subunit I (CO1) gene, partial cds; mitochondrial	504	88.4%	672.479	0.00e+00	91.9%
15	OQ530222	Phoridae sp. voucher ZRCBDP0364586 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	676.444	0.00e+00	91.3%
16	OQ530431	Phoridae sp. voucher ZRCBDP0364825 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	676.444	0.00e+00	91.3%
17	OQ530428	Phoridae sp. voucher ZRCBDP0364822 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	668.515	0.00e+00	91.1%
18	MN403778	Phoridae sp. isolate UGC0005429 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	531	93.2%	654.639	0.00e+00	90.6%
19	MN404130	Phoridae sp. isolate UGC0006441 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	531	93.2%	654.639	0.00e+00	90.6%
20	OQ532738	Phoridae sp. voucher ZRCBDP364248 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	644.728	3.84e-180	90.5%
21	JN298710	Diptera sp. BOLD:AAU5601 voucher gvc14959-1L cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	650.674	0.00e+00	90.4%
22	MN407575	Phoridae sp. isolate UGC0005413 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	531	93.2%	648.692	0.00e+00	90.4%
23	MN404062	Phoridae sp. isolate UGC0004728 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	531	93.2%	648.692	0.00e+00	90.4%
24	MG290834	Megaselia rufipes voucher BIOUG27635-D02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	642.745	1.52e-179	90.4%
25	MN409852	Phoridae sp. isolate UGC0003246 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
26	MN407428	Phoridae sp. isolate UGC0006415 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
27	MN403995	Phoridae sp. isolate UGC0009773 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
28	MN403516	Phoridae sp. isolate UGC0011929 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
29	MN406576	Phoridae sp. isolate UGC0002271 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
30	MN407279	Phoridae sp. isolate UGC0012168 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
31	MN404305	Phoridae sp. isolate UGC0011652 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
32	MN409697	Phoridae sp. isolate UGC0002241 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
33	MN405774	Phoridae sp. isolate UGC0003384 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
34	MN405262	Phoridae sp. isolate UGC0012385 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
35	MN404154	Phoridae sp. isolate UGC0012139 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
36	MN405370	Phoridae sp. isolate UGC0002254 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
37	MN404155	Phoridae sp. isolate UGC0011117 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
38	MN404482	Phoridae sp. isolate UGC0002236 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	636.798	9.35e-178	90.4%
39	MF867663	Megaselia nigriceps voucher BIOUG21044-C01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	510	89.5%	620.94	5.56e-173	90.4%
40	KT702116	Phoridae sp. BOLD:AAM9378 voucher BIOUG22634-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	510	89.5%	620.94	5.56e-173	90.4%
41	KT100231	Megaselia sp. BOLD-2016 voucher BIOUG02891-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	510	89.5%	620.94	5.56e-173	90.4%
42	KT111453	Megaselia sp. BOLD-2016 voucher BIOUG01307-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	636.798	9.35e-178	90.3%
43	MN408458	Phoridae sp. isolate UGC0002106 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	634.816	3.70e-177	90.3%
44	MN408962	Phoridae sp. isolate UGC0012916 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	634.816	3.70e-177	90.3%
45	MF863572	Megaselia nigriceps voucher BIOUG21860-H04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	504	88.4%	609.047	2.11e-169	90.3%
46	MG297140	Megaselia rufipes voucher BIOUG30415-D04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	636.798	9.35e-178	90.2%
47	KR682983	Megaselia rufipes voucher BIOUG01756-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	636.798	9.35e-178	90.2%
48	KR678660	Megaselia rufipes voucher BIOUG01752-D12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	636.798	9.35e-178	90.2%
49	MG296924	Megaselia rufipes voucher BIOUG27637-H02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	634.816	3.70e-177	90.2%
50	MN407450	Phoridae sp. isolate UGC0005080 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	630.852	5.77e-176	90.2%
51	MN404682	Phoridae sp. isolate UGC0012412 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	630.852	5.77e-176	90.2%
52	MN406060	Phoridae sp. isolate UGC0005352 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	628.869	2.28e-175	90.2%
53	KR644500	Megaselia sp. BOLD-2016 voucher BIOUG02514-D11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	510	89.5%	614.994	3.43e-171	90.2%
54	KT108478	Megaselia sp. BOLD-2016 voucher BIOUG01307-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	510	89.5%	613.011	1.35e-170	90.2%
55	KR756045	Megaselia sp. BOLD-2016 voucher 10PHMAL-2819 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	510	89.5%	613.011	1.35e-170	90.2%
56	MN409868	Phoridae sp. isolate UGC0002123 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	630.852	5.77e-176	90.1%
57	KR637343	Phoridae sp. BOLD-2016 voucher BIOUG02724-F10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
58	KR740358	Megaselia sp. BOLD-2016 voucher 10PHMAL-1232 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
59	KR745674	Megaselia sp. BOLD-2016 voucher BIOUG01298-F05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
60	KT702626	Phoridae sp. BOLD:AAM9378 voucher BIOUG22835-C05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
61	MN409850	Phoridae sp. isolate UGC0003245 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	628.869	2.28e-175	90.1%
62	OQ532605	Phoridae sp. voucher ZRCBDP0375694 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
63	MF862875	Megaselia nigriceps voucher BIOUG21845-A07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
64	MG102796	Megaselia nigriceps voucher BIOUG01423-H03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
65	KT703952	Phoridae sp. BOLD:AAM9378 voucher BIOUG22634-E04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
66	KT704583	Phoridae sp. BOLD:AAM9378 voucher BIOUG22634-F04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
67	MN407798	Phoridae sp. isolate UGC0010250 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	628.869	2.28e-175	90.1%
68	HQ982263	Megaselia nigriceps voucher BIOUG	526	92.3%	628.869	2.28e-175	90.1%
69	KR665553	Megaselia sp. BOLD-2016 voucher BIOUG07773-H04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
70	KR646358	Megaselia sp. BOLD-2016 voucher BIOUG02628-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	628.869	2.28e-175	90.1%
71	MN404930	Phoridae sp. isolate UGC0012558 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	628.869	2.28e-175	90.1%
72	MN404454	Phoridae sp. isolate UGC0012846 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	626.887	9.01e-175	90.1%
73	MN408050	Phoridae sp. isolate UGC0012855 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	626.887	9.01e-175	90.1%
74	MN407339	Phoridae sp. isolate UGC0012835 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	626.887	9.01e-175	90.1%
75	MN406924	Phoridae sp. isolate UGC0004794 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	620.94	5.56e-173	90.1%
76	MN403623	Phoridae sp. isolate UGC0004782 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	620.94	5.56e-173	90.1%
77	MN409854	Phoridae sp. isolate UGC0003241 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	620.94	5.56e-173	90.1%
78	MN409527	Phoridae sp. isolate UGC0010938 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	620.94	5.56e-173	90.1%
79	MF855192	Diplonevra nitidula voucher BIOUG21367-B01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	513	90.0%	611.029	5.35e-170	90.1%
80	KJ085265	Megaselia sp. BOLD:AAG3274 voucher BIOUG03245-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
81	KJ085595	Megaselia sp. BOLD:AAG3274 voucher BIOUG03248-A11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
82	KJ167009	Megaselia sp. BOLD:AAG3274 voucher BIOUG03369-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
83	HM417219	Megaselia hardingorum voucher BIOUG	530	93.0%	628.869	2.28e-175	90.0%
84	KJ091604	Megaselia sp. BOLD:AAG3274 voucher BIOUG03246-D10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
85	KJ084091	Megaselia sp. BOLD:AAG3274 voucher BIOUG03369-E08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
86	KR679085	Megaselia rufipes voucher BIOUG01672-A06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
87	KJ086321	Megaselia sp. BOLD:AAG3274 voucher BIOUG03261-A03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
88	KR677841	Megaselia rufipes voucher BIOUG01670-B10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	628.869	2.28e-175	90.0%
89	MG102877	Megaselia rufipes voucher BIOUG27398-C08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	626.887	9.01e-175	90.0%
90	MG296376	Megaselia rufipes voucher BIOUG30431-D11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	626.887	9.01e-175	90.0%
91	MG102653	Megaselia rufipes voucher BIOUG25761-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	626.887	9.01e-175	90.0%
92	MG110429	Megaselia rufipes voucher BIOUG27526-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	626.887	9.01e-175	90.0%
93	MF865818	Megaselia rufipes voucher BIOUG21467-E08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	626.887	9.01e-175	90.0%
94	KR764070	Diplonevra nitidula voucher BIOUG16051-E03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	620.94	5.56e-173	90.0%
95	KT095085	Megaselia rufipes voucher BIOUG09307-G11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	521	91.4%	618.958	2.20e-172	90.0%
96	KR757112	Megaselia rufipes voucher BIOUG05716-G09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	520	91.2%	616.976	8.67e-172	90.0%
97	KT700267	Phoridae sp. BOLD:AAM9378 voucher BIOUG22847-A03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	520	91.2%	616.976	8.67e-172	90.0%
98	KT705118	Phoridae sp. BOLD:AAM9378 voucher BIOUG22842-D11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	520	91.2%	616.976	8.67e-172	90.0%
99	MG108157	Diplonevra nitidula voucher BIOUG08693-G12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	511	89.6%	607.065	8.35e-169	90.0%
100	KR751078	Megaselia sp. BOLD-2016 voucher BIOUG01580-C09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	510	89.5%	605.082	3.30e-168	90.0%
101	JF871662	Phoridae sp. BOLD:AAM9348 voucher BIOUG	533	93.5%	626.887	9.01e-175	89.9%
102	OR929511	Megaselia flava voucher SMNS_1199903 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	626.887	9.01e-175	89.9%
103	KJ092156	Phoridae sp. BOLD:AAM9378 voucher BIOUG03341-D09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
104	OQ528033	Phoridae sp. voucher ZRCBDP0361597 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
105	KR429305	Megaselia sp. BOLD-2016 voucher BIOUG04314-C07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
106	KT100851	Diplonevra nitidula voucher BIOUG01360-G09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
107	KR643382	Megaselia sp. BOLD-2016 voucher BIOUG02665-C01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
108	KR637687	Phoridae sp. BOLD-2016 voucher BIOUG02669-C06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
109	HQ581779	Megaselia nigriceps voucher BIOUG	526	92.3%	620.94	5.56e-173	89.9%
110	OR924481	Diplonevra nitidula voucher SMNS_1206456 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
111	MG105857	Megaselia nigriceps voucher BIOUG23941-H01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
112	MN408455	Phoridae sp. isolate UGC0002103 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	620.94	5.56e-173	89.9%
113	MN407049	Phoridae sp. isolate UGC0013393 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	620.94	5.56e-173	89.9%
114	KR438019	Megaselia sp. BOLD-2016 voucher BIOUG04313-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
115	KT703344	Phoridae sp. BOLD:AAM9378 voucher BIOUG22634-D09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
116	MN409350	Phoridae sp. isolate UGC0013322 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	620.94	5.56e-173	89.9%
117	KR747476	Megaselia sp. BOLD-2016 voucher 10PHMAL-1325 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
118	MN407035	Phoridae sp. isolate UGC0012624 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	620.94	5.56e-173	89.9%
119	KJ163851	Phoridae sp. BOLD:AAM9378 voucher BIOUG03448-A03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
120	MF862353	Megaselia nigriceps voucher BIOUG21008-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	620.94	5.56e-173	89.9%
121	MN409333	Phoridae sp. isolate UGC0002160 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	618.958	2.20e-172	89.9%
122	MN408836	Phoridae sp. isolate UGC0012564 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	618.958	2.20e-172	89.9%
123	MN407234	Phoridae sp. isolate UGC0013187 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	618.958	2.20e-172	89.9%
124	MN405556	Phoridae sp. isolate UGC0002146 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	618.958	2.20e-172	89.9%
125	MN409619	Phoridae sp. isolate UGC0002143 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	618.958	2.20e-172	89.9%
126	MN408105	Phoridae sp. isolate UGC0013066 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	616.976	8.67e-172	89.9%
127	MN404363	Phoridae sp. isolate UGC0004793 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.9%
128	MN410201	Phoridae sp. isolate UGC0004785 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.9%
129	MG108638	Megaselia nigriceps voucher BIOUG23941-G04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
130	KT098245	Diplonevra nitidula voucher BIOUG10426-C06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
131	KR652822	Diplonevra nitidula voucher BIOUG02722-G08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
132	KT113349	Megaselia sp. BOLD-2016 voucher BIOUG02907-G10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
133	MF861847	Megaselia rufipes voucher BIOUG26464-F03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
134	KR638887	Diplonevra nitidula voucher BIOUG02640-F02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
135	KT087777	Diplonevra nitidula voucher BIOUG10420-A01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
136	KT086540	Diplonevra nitidula voucher BIOUG11003-F07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
137	KT106395	Megaselia sp. BOLD-2016 voucher BIOUG02868-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	517	90.7%	611.029	5.35e-170	89.9%
138	KR432130	Diplonevra nitidula voucher BIOUG04480-E10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	515	90.4%	607.065	8.35e-169	89.9%
139	KR641417	Megaselia rufipes voucher BIOUG02600-G06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
140	KY842168	Phoridae sp. BIOUG02131-B04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
141	KY837331	Phoridae sp. BIOUG02131-G06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
142	KR651910	Megaselia rufipes voucher BIOUG02652-F07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
143	KR639801	Phoridae sp. BOLD-2016 voucher BIOUG02596-G09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
144	MG294878	Megaselia rufipes voucher BIOUG30431-B06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
145	KR647916	Megaselia rufipes voucher BIOUG02597-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
146	KR642899	Megaselia rufipes voucher BIOUG02599-H03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
147	KY844607	Phoridae sp. BIOUG02132-A11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
148	KR639933	Megaselia sp. BOLD-2016 voucher BIOUG02599-C12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
149	KR655094	Megaselia rufipes voucher BIOUG09353-D09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
150	KR682416	Megaselia rufipes voucher BIOUG01672-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
151	KR662704	Megaselia rufipes voucher BIOUG09139-E04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	620.94	5.56e-173	89.8%
152	KP049684	Phoridae sp. BOLD:AAP8722 voucher BIOUG04642-E07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	618.958	2.20e-172	89.8%
153	KT622638	Megaselia rufipes voucher BIOUG21483-A02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	618.958	2.20e-172	89.8%
154	MF863309	Megaselia rufipes voucher BIOUG21648-B12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	618.958	2.20e-172	89.8%
155	MN407448	Phoridae sp. isolate UGC0004755 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	529	92.8%	618.958	2.20e-172	89.8%
156	MF864881	Megaselia sp. BIOUG26984-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	616.976	8.67e-172	89.8%
157	MN671753	Megaselia shatesae voucher CHARS00215-D12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	527	92.5%	614.994	3.43e-171	89.8%
158	KT111736	Diplonevra nitidula voucher BIOUG02891-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
159	KR773053	Diplonevra nitidula voucher BIOUG08380-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
160	MN682895	Megaselia shatesae voucher CHARS00227-E12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.8%
161	KR768070	Diplonevra nitidula voucher BIOUG16037-F04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
162	KR763321	Diplonevra nitidula voucher BIOUG16051-C07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
163	KR758903	Diplonevra nitidula voucher BIOUG08662-B03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
164	KR758888	Diplonevra nitidula voucher BIOUG08684-G10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
165	KT704808	Phoridae sp. BOLD:AAG3236 voucher BIOUG22721-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
166	GU806506	Diplonevra nitidula voucher BIOUG	522	91.6%	613.011	1.35e-170	89.8%
167	KR767527	Diplonevra nitidula voucher BIOUG16090-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
168	MG108077	Diplonevra nitidula voucher BIOUG24730-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
169	KT702809	Phoridae sp. BOLD:AAG3236 voucher BIOUG22865-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
170	MN672926	Megaselia shatesae voucher CHARS00145-B12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.8%
171	MG293787	Phorinae sp. BIOUG31124-B11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
172	MN676919	Megaselia shatesae voucher CHARS00239-D03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.8%
173	KT111909	Diplonevra nitidula voucher BIOUG02874-B09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
174	KT077973	Diplonevra nitidula voucher BIOUG10495-E09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
175	KR519134	Diplonevra nitidula voucher BIOUG12661-D06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
176	KR667507	Diplonevra nitidula voucher BIOUG07710-G12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
177	KT094493	Diplonevra nitidula voucher BIOUG08713-H11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
178	MG109460	Diplonevra nitidula voucher BIOUG25588-B09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
179	KT119243	Diplonevra nitidula voucher BIOUG02905-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
180	KR767290	Diplonevra nitidula voucher BIOUG08495-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
181	MN676109	Megaselia shatesae voucher CHARS00229-B04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.8%
182	KR768081	Diplonevra nitidula voucher BIOUG08380-D09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
183	MG294999	Phorinae sp. BIOUG31153-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
184	HM412839	Diplonevra nitidula voucher BIOUG	522	91.6%	613.011	1.35e-170	89.8%
185	KT100418	Diplonevra nitidula voucher BIOUG02895-F10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
186	KT095728	Diplonevra nitidula voucher BIOUG12168-B11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
187	KR521252	Diplonevra nitidula voucher BIOUG13705-B10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
188	KT115261	Diplonevra nitidula voucher BIOUG02896-G06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
189	KT093727	Diplonevra nitidula voucher BIOUG12165-C12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	613.011	1.35e-170	89.8%
190	MN676082	Megaselia shatesae voucher CHARS00146-F02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.8%
191	MG296563	Megaselia rufipes voucher BIOUG27568-C06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	520	91.2%	609.047	2.11e-169	89.8%
192	MF867576	Megaselia nigriceps voucher BIOUG21178-E10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	520	91.2%	609.047	2.11e-169	89.8%
193	MG107139	Megaselia rufipes voucher BIOUG27399-A10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	520	91.2%	609.047	2.11e-169	89.8%
194	KR644384	Diplonevra nitidula voucher BIOUG02499-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	519	91.1%	607.065	8.35e-169	89.8%
195	KR654224	Diplonevra nitidula voucher BIOUG08937-H01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
196	KT084997	Diplonevra nitidula voucher BIOUG11244-H05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
197	KR654355	Diplonevra nitidula voucher BIOUG09133-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
198	KR665481	Diplonevra nitidula voucher BIOUG10069-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
199	KR657282	Diplonevra nitidula voucher BIOUG09135-H04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
200	KT092390	Diplonevra nitidula voucher BIOUG11245-G11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
201	KT086600	Diplonevra nitidula voucher BIOUG10549-C04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
202	KP044154	Diplonevra nitidula voucher BIOUG06889-C02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	518	90.9%	605.082	3.30e-168	89.8%
203	KY838916	Phoridae sp. BIOUG04901-G09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	618.958	2.20e-172	89.7%
204	KY846810	Phoridae sp. BIOUG04902-D02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	618.958	2.20e-172	89.7%
205	KY843331	Phoridae sp. BIOUG04902-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	618.958	2.20e-172	89.7%
206	KR429261	Megaselia sp. BOLD-2016 voucher BIOUG05824-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	618.958	2.20e-172	89.7%
207	KY830598	Phoridae sp. BIOUG04901-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	618.958	2.20e-172	89.7%
208	KR681263	Megaselia sp. BOLD-2016 voucher BIOUG07176-B07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	618.958	2.20e-172	89.7%
209	HQ582861	Phoridae sp. BOLD:AAM9348 voucher BIOUG	533	93.5%	618.958	2.20e-172	89.7%
210	KY839018	Phoridae sp. BIOUG04902-D11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	618.958	2.20e-172	89.7%
211	KR990760	Megaselia sp. BOLD-2016 voucher BIOUG19332-B04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	616.976	8.67e-172	89.7%
212	KR988489	Megaselia sp. BOLD-2016 voucher BIOUG19256-D04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	616.976	8.67e-172	89.7%
213	MN409993	Phoridae sp. isolate UGC0013113 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	614.994	3.43e-171	89.7%
214	MF867568	Megaselia nigriceps voucher BIOUG21008-B02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	614.994	3.43e-171	89.7%
215	KR743423	Diplonevra nitidula voucher BIOUG10772-F02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
216	KR637697	Phoridae sp. BOLD-2016 voucher BIOUG02722-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
217	KR639091	Phoridae sp. BOLD-2016 voucher BIOUG02722-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
218	KR638552	Phoridae sp. BOLD-2016 voucher BIOUG02640-E02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
219	MN403637	Phoridae sp. isolate UGC0013177 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	613.011	1.35e-170	89.7%
220	KR772021	Diplonevra nitidula voucher BIOUG16098-E10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
221	KR646722	Diplonevra nitidula voucher BIOUG02656-D10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
222	KT076289	Diplonevra nitidula voucher BIOUG09937-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
223	KR761595	Diplonevra nitidula voucher BIOUG16001-C05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
224	KR756246	Diplonevra nitidula voucher 10PHMAL-1194 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
225	KR666997	Diplonevra nitidula voucher BIOUG09139-B08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
226	KR749576	Diplonevra nitidula voucher BIOUG10726-F11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
227	KR673233	Diplonevra nitidula voucher BIOUG09003-B04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
228	HM386323	Diplonevra nitidula voucher BIOUG	526	92.3%	613.011	1.35e-170	89.7%
229	KT095099	Diplonevra nitidula voucher BIOUG11146-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
230	KJ085038	Diplonevra nitidula voucher BIOUG03249-A01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
231	KT089649	Diplonevra nitidula voucher BIOUG11224-F07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
232	KR633434	Diplonevra nitidula voucher BIOUG02384-E10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
233	KR756325	Diplonevra nitidula voucher BIOUG10772-E12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
234	KR672854	Diplonevra nitidula voucher BIOUG08561-D01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
235	KR655364	Diplonevra nitidula voucher BIOUG10069-F07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
236	KR640418	Phoridae sp. BOLD-2016 voucher BIOUG02499-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
237	KT112892	Diplonevra nitidula voucher BIOUG02929-D12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
238	KR639911	Phoridae sp. BOLD-2016 voucher BIOUG02640-A10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
239	KR740903	Diplonevra nitidula voucher 10PHMAL-1247 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
240	KR646266	Diplonevra nitidula voucher BIOUG02499-E07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
241	KT095270	Diplonevra nitidula voucher BIOUG11085-C06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
242	KT075529	Diplonevra nitidula voucher BIOUG10709-F02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
243	MG102558	Phorinae sp. BIOUG32188-C03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
244	KT087001	Diplonevra nitidula voucher BIOUG11018-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
245	KR425977	Diplonevra nitidula voucher BIOUG04314-F04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
246	HQ577725	Diplonevra nitidula voucher BIOUG	526	92.3%	613.011	1.35e-170	89.7%
247	KR671340	Diplonevra nitidula voucher BIOUG09140-G06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
248	HQ981766	Diplonevra nitidula voucher BIOUG	526	92.3%	613.011	1.35e-170	89.7%
249	KR640581	Phoridae sp. BOLD-2016 voucher BIOUG02630-B10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
250	KT093370	Diplonevra nitidula voucher BIOUG10385-B05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
251	KT081707	Diplonevra nitidula voucher BIOUG11088-C03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
252	KR655682	Diplonevra nitidula voucher BIOUG01604-D04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
253	MG109992	Megaselia nigriceps voucher BIOUG25523-C01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
254	KT082282	Diplonevra nitidula voucher BIOUG09996-E07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
255	JN302057	Diplonevra nitidula voucher BIOUG	526	92.3%	613.011	1.35e-170	89.7%
256	KR649655	Diplonevra nitidula voucher BIOUG07126-G08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
257	KT080659	Diplonevra nitidula voucher BIOUG10783-D10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
258	KT096188	Diplonevra nitidula voucher BIOUG11223-B01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
259	KR441354	Diplonevra nitidula voucher BIOUG06079-H03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
260	KR641217	Diplonevra nitidula voucher BIOUG02656-C12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
261	KP043490	Diplonevra nitidula voucher BIOUG06890-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
262	KR653611	Diplonevra nitidula voucher BIOUG09930-B03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
263	KJ208317	Diplonevra nitidula voucher BIOUG03908-H07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
264	KP039191	Diplonevra nitidula voucher BIOUG07024-E09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
265	KR431128	Diplonevra nitidula voucher BIOUG06074-A07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
266	KR686736	Diplonevra nitidula voucher BIOUG01796-F05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
267	MG294101	Phorinae sp. BIOUG32604-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
268	KT081983	Diplonevra nitidula voucher BIOUG10420-B10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
269	KR761563	Diplonevra nitidula voucher BIOUG16038-C01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
270	KR432059	Diplonevra nitidula voucher BIOUG06081-A08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
271	KR641200	Diplonevra nitidula voucher BIOUG02384-H01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
272	OQ532359	Phoridae sp. voucher ZRCBDP0375434 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
273	KR639519	Phoridae sp. BOLD-2016 voucher BIOUG02656-C08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
274	KR652037	Diplonevra nitidula voucher BIOUG02722-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
275	KR752171	Diplonevra nitidula voucher BIOUG01485-C02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
276	KR752075	Diplonevra nitidula voucher 10PHMAL-1195 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
277	KR748213	Diplonevra nitidula voucher 10PHMAL-1238 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
278	KR749383	Diplonevra nitidula voucher 10PHMAL-1212 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
279	KR641258	Diplonevra nitidula voucher BIOUG02384-F12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
280	KR660196	Diplonevra nitidula voucher BIOUG09135-B09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
281	KR643036	Diplonevra nitidula voucher BIOUG02722-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
282	KR644885	Diplonevra nitidula voucher BIOUG02722-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
283	KT096039	Diplonevra nitidula voucher BIOUG11003-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
284	KR657703	Diplonevra nitidula voucher BIOUG09134-B05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
285	KT089820	Diplonevra nitidula voucher BIOUG11150-B09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
286	KT076460	Diplonevra nitidula voucher BIOUG11245-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
287	KT092197	Diplonevra nitidula voucher BIOUG11244-B08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
288	KR644796	Diplonevra nitidula voucher BIOUG02722-G12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
289	KR775653	Diplonevra nitidula voucher BIOUG16101-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
290	KR778475	Diplonevra nitidula voucher BIOUG16079-D10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
291	KT083420	Diplonevra nitidula voucher BIOUG11004-D06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
292	KT086470	Diplonevra nitidula voucher BIOUG11017-C04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
293	KR770123	Diplonevra nitidula voucher BIOUG16075-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
294	KR649481	Diplonevra nitidula voucher BIOUG02499-E02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
295	KR647780	Diplonevra nitidula voucher BIOUG02656-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
296	KR744952	Diplonevra nitidula voucher 10PHMAL-1191 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
297	KR636264	Diplonevra nitidula voucher BIOUG02640-C08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
298	KR642625	Diplonevra nitidula voucher BIOUG02722-F12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
299	KT706013	Phoridae sp. BOLD:AAG3236 voucher BIOUG24009-H02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
300	KR640813	Phoridae sp. BOLD-2016 voucher BIOUG02713-H01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
301	KT090538	Diplonevra nitidula voucher BIOUG11004-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
302	KT118429	Diplonevra nitidula voucher BIOUG02903-E03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
303	OR924480	Diplonevra nitidula voucher SMNS_1197059 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
304	MN410292	Phoridae sp. isolate UGC0013139 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	525	92.1%	613.011	1.35e-170	89.7%
305	KP044005	Diplonevra nitidula voucher BIOUG07024-G11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
306	MG291912	Phorinae sp. BIOUG32602-B10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
307	KR640473	Phoridae sp. BOLD-2016 voucher BIOUG02656-D04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
308	KT077187	Diplonevra nitidula voucher BIOUG09956-B11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
309	KT081228	Diplonevra nitidula voucher BIOUG11066-E09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
310	KT106226	Diplonevra nitidula voucher BIOUG01143-C01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
311	KP043035	Diplonevra nitidula voucher BIOUG03996-D01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
312	KP043609	Diplonevra nitidula voucher BIOUG03838-H05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
313	MZ608729	Diplonevra nitidula voucher MZH_GV.3216 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
314	KR665062	Diplonevra nitidula voucher BIOUG09135-A06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
315	OQ530649	Phoridae sp. voucher ZRCBDP0365085 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
316	KR430762	Diplonevra nitidula voucher BIOUG06079-H04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
317	HQ979148	Phoridae sp. BOLD:AAG3236 voucher BIOUG	526	92.3%	613.011	1.35e-170	89.7%
318	KR660010	Diplonevra nitidula voucher BIOUG09634-H05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
319	KR658987	Diplonevra nitidula voucher BIOUG08933-C03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
320	KR652540	Diplonevra nitidula voucher BIOUG02640-H11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
321	KR663000	Diplonevra nitidula voucher BIOUG10068-B02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
322	KT086165	Diplonevra nitidula voucher BIOUG14407-G12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
323	KT085250	Phoridae sp. BOLD-2016 voucher BIOUG13375-F11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
324	KR672540	Diplonevra nitidula voucher BIOUG09345-B01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	613.011	1.35e-170	89.7%
325	MN403866	Phoridae sp. isolate UGC0004749 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	522	91.6%	607.065	8.35e-169	89.7%
326	MG291856	Diplonevra nitidula voucher BIOUG27568-F08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	607.065	8.35e-169	89.7%
327	OQ530375	Phoridae sp. voucher ZRCBDP0364753 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	522	91.6%	605.082	3.30e-168	89.7%
328	KR770630	Diplonevra nitidula voucher BIOUG08569-A09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	522	91.6%	605.082	3.30e-168	89.7%
329	OQ532278	Phoridae sp. voucher ZRCBDP0375351 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	536	94.0%	616.976	8.67e-172	89.6%
330	OQ530542	Phoridae sp. voucher ZRCBDP0364952 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	536	94.0%	616.976	8.67e-172	89.6%
331	KR438592	Megaselia sp. BOLD:AAG3274 voucher BIOUG04290-A04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
332	KJ444045	Megaselia sp. BOLD:AAG3274 voucher BIOUG03749-D01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
333	KR639330	Phoridae sp. BOLD-2016 voucher BIOUG02648-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
334	OR930854	Megaselia perfusca voucher SMNS_1203843 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
335	KR649217	Megaselia rufipes voucher BIOUG02596-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
336	KU873828	Phoridae sp. UAM Ento 234170 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
337	JF867966	Megaselia sp. BBDCN446-10 voucher BIOUG	530	93.0%	613.011	1.35e-170	89.6%
338	KR650584	Megaselia rufipes voucher BIOUG02650-B05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
339	KT117235	Megaselia rufipes voucher BIOUG01434-E05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
340	KT075944	Megaselia rufipes voucher BIOUG10528-B08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
341	KR633724	Megaselia rufipes voucher BIOUG02647-E11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
342	KT116952	Megaselia rufipes voucher BIOUG01126-C12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	613.011	1.35e-170	89.6%
343	KM646622	Phoridae sp. BOLD:AAM9371 voucher BIOUG06748-C12 cytochrome oxidase subunit 1 (COI) gene, promoter region and partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
344	KM907418	Phoridae sp. BOLD:AAP8722 voucher BIOUG06353-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
345	KM955196	Phoridae sp. BOLD:AAP8722 voucher BIOUG06504-H11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
346	KT088267	Phoridae sp. BOLD-2016 voucher BIOUG10714-B06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
347	KM962462	Phoridae sp. BOLD:AAM9371 voucher BIOUG06841-C07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
348	KC502706	Phoridae sp. BOLD:AAP8722 voucher CCDB-08111-E12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
349	KP039542	Phoridae sp. BOLD:AAP8722 voucher BIOUG06431-G11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
350	KM639260	Phoridae sp. BOLD:AAP8722 voucher BIOUG09582-H01 cytochrome oxidase subunit 1 (COI) gene, promoter region and partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
351	KM964472	Phoridae sp. BOLD:AAP8722 voucher BIOUG06504-F10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
352	KC502714	Phoridae sp. BOLD:AAP8722 voucher CCDB-08111-F12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
353	KR430401	Megaselia sp. BOLD-2016 voucher BIOUG04301-E10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
354	JF875550	Megaselia sp. JWDCF776-10 voucher BIOUG	529	92.8%	611.029	5.35e-170	89.6%
355	KM967341	Phoridae sp. BOLD:AAP8722 voucher BIOUG06504-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
356	KR430286	Megaselia sp. BOLD-2016 voucher BIOUG03572-F02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
357	KM913290	Phoridae sp. BOLD:AAM9371 voucher BIOUG06908-C01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
358	KM941011	Phoridae sp. BOLD:AAP8722 voucher BIOUG04205-F07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
359	KM957436	Phoridae sp. BOLD:AAP8722 voucher BIOUG06512-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
360	KM956973	Phoridae sp. BOLD:AAM9371 voucher BIOUG06897-C04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
361	KM914472	Phoridae sp. BOLD:AAM9371 voucher BIOUG06910-H11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
362	KC502695	Phoridae sp. BOLD:AAP8722 voucher CCDB-08111-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
363	KP039463	Phoridae sp. BOLD:AAP8722 voucher BIOUG06530-G06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
364	KR750805	Megaselia rufipes voucher BIOUG05719-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
365	KM967693	Phoridae sp. BOLD:AAP8722 voucher BIOUG06504-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
366	KM900515	Phoridae sp. BOLD:AAP8722 voucher BIOUG09620-D02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
367	JF875205	Megaselia sp. JWDCF394-10 voucher BIOUG	529	92.8%	611.029	5.35e-170	89.6%
368	KX054407	Phoridae sp. sc_06909 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
369	KM629267	Phoridae sp. BOLD:AAM9371 voucher BIOUG06748-A05 cytochrome oxidase subunit 1 (COI) gene, promoter region and partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
370	MF861454	Megaselia sp. BIOUG26982-G07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
371	KR745677	Megaselia rufipes voucher BIOUG05719-H08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
372	KP049362	Phoridae sp. BOLD:AAP8722 voucher BIOUG06531-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
373	KR472260	Megaselia sp. BOLD-2016 voucher BIOUG03791-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
374	MN408445	Phoridae sp. isolate UGC0003372 cytochrome oxidase subunit I (cox1) gene, partial cds; mitochondrial	528	92.6%	611.029	5.35e-170	89.6%
375	KM919941	Phoridae sp. BOLD:AAP8722 voucher BIOUG09341-A02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
376	KR439173	Phalacrotophora sp. BOLD-2016 voucher BIOUG02056-H09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
377	KM629829	Megaselia sp. BOLD:AAG3274 voucher BIOUG06370-B07 cytochrome oxidase subunit 1 (COI) gene, promoter region and partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
378	KM944990	Phoridae sp. BOLD:AAP8722 voucher BIOUG04169-A12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
379	KR986669	Megaselia sp. BOLD-2016 voucher BIOUG19714-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
380	KM915656	Phoridae sp. BOLD:AAP8722 voucher BIOUG08706-E03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
381	KR430192	Phalacrotophora sp. BOLD-2016 voucher BIOUG02056-H05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
382	KT098214	Megaselia rufipes voucher BIOUG11431-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
383	KR444991	Megaselia sp. BOLD-2016 voucher BIOUG04026-D12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
384	KR466098	Megaselia sp. BOLD-2016 voucher BIOUG03794-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
385	MG105315	Megaselia sp. BIOUG05657-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
386	KR473757	Megaselia sp. BOLD-2016 voucher BIOUG03868-A07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
387	KM917399	Phoridae sp. BOLD:AAP8722 voucher BIOUG07090-F09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	529	92.8%	611.029	5.35e-170	89.6%
388	KR603257	Megaselia sp. BOLD-2016 voucher BIOUG17504-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
389	KR622976	Megaselia sp. BOLD-2016 voucher BIOUG16922-D03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
390	MG103977	Megaselia sp. BIOUG24208-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
391	MG107482	Megaselia sp. BIOUG22905-B04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
392	MF870636	Megaselia sp. BIOUG27574-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
393	MF861858	Megaselia sp. BIOUG26988-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
394	MG104608	Megaselia sp. BIOUG24369-F03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
395	KR598368	Megaselia sp. BOLD-2016 voucher BIOUG17563-A03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
396	KM918713	Phoridae sp. BOLD:AAP8722 voucher BIOUG09205-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
397	MG109557	Megaselia sp. BIOUG22904-E07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
398	MG110698	Megaselia sp. BIOUG22802-B02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
399	KR586255	Megaselia sp. BOLD-2016 voucher BIOUG17586-H02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
400	KT085792	Phoridae sp. BOLD-2016 voucher BIOUG12368-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
401	KR990214	Megaselia sp. BOLD-2016 voucher BIOUG19687-A01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
402	MF865074	Megaselia sp. BIOUG26987-D10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
403	KR991152	Megaselia sp. BOLD-2016 voucher BIOUG19226-E12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
404	MN681761	Megaselia shatesae voucher CHARS00145-G11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	528	92.6%	609.047	2.11e-169	89.6%
405	MN676798	Megaselia shatesae voucher CHARS00257-C05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	527	92.5%	607.065	8.35e-169	89.6%
406	MN683300	Megaselia shatesae voucher CHARS00263-F03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	527	92.5%	607.065	8.35e-169	89.6%
407	MN675193	Megaselia shatesae voucher CHARS00204-B10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	527	92.5%	607.065	8.35e-169	89.6%
408	MN668567	Megaselia shatesae voucher CHARS00205-B01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	527	92.5%	607.065	8.35e-169	89.6%
409	MN679513	Megaselia shatesae voucher CHARS00147-A10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	527	92.5%	607.065	8.35e-169	89.6%
410	MN671140	Megaselia shatesae voucher CHARS00238-H05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
411	MN682253	Megaselia shatesae voucher CHARS00238-H01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
412	MN666018	Megaselia shatesae voucher CHARS00254-E04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
413	MN677277	Megaselia shatesae voucher CHARS00205-E09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
414	MN672220	Megaselia shatesae voucher CHARS00264-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
415	MN676773	Megaselia shatesae voucher CHARS00259-A09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
416	MN680004	Megaselia shatesae voucher CHARS00229-A10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
417	MN677892	Megaselia shatesae voucher CHARS00228-C08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
418	MN666320	Megaselia shatesae voucher CHARS00206-A10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
419	MN668217	Megaselia shatesae voucher CHARS00278-H02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
420	MN672076	Megaselia shatesae voucher CHARS00146-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.6%
421	KU873821	Phoridae sp. UAM Ento 232440 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	616.976	8.67e-172	89.5%
422	KR692294	Megaselia sp. BOLD-2016 voucher BIOUG07793-A02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	611.029	5.35e-170	89.5%
423	OQ529228	Phoridae sp. voucher ZRCBDP0362951 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	533	93.5%	611.029	5.35e-170	89.5%
424	KR426705	Megaselia sp. BOLD-2016 voucher BIOUG05769-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	611.029	5.35e-170	89.5%
425	KR691549	Megaselia sp. BOLD-2016 voucher BIOUG07117-A10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	611.029	5.35e-170	89.5%
426	KM938224	Megaselia sp. BOLD:ACA8350 voucher BIOUG07069-H08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	611.029	5.35e-170	89.5%
427	KU873824	Phoridae sp. UAM Ento 232665 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	533	93.5%	611.029	5.35e-170	89.5%
428	KR467457	Megaselia sp. BOLD-2016 voucher BIOUG18507-D02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	609.047	2.11e-169	89.5%
429	MF852870	Megaselia sp. BIOUG21727-C04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	609.047	2.11e-169	89.5%
430	KR991645	Megaselia sp. BOLD-2016 voucher BIOUG19257-C02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	609.047	2.11e-169	89.5%
431	KM628808	Phoridae sp. BOLD:ACB9084 voucher BIOUG04817-D02 cytochrome oxidase subunit 1 (COI) gene, promoter region and partial cds; mitochondrial	532	93.3%	609.047	2.11e-169	89.5%
432	KR991779	Megaselia sp. BOLD-2016 voucher BIOUG19257-F10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	609.047	2.11e-169	89.5%
433	MF854698	Megaselia sp. BIOUG22158-E10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	609.047	2.11e-169	89.5%
434	MF857028	Megaselia sp. BIOUG21727-E05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	532	93.3%	609.047	2.11e-169	89.5%
435	MG108946	Megaselia sp. BIOUG22909-C06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	531	93.2%	607.065	8.35e-169	89.5%
436	KR502811	Megaselia sp. BOLD-2016 voucher BIOUG18591-A11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	531	93.2%	607.065	8.35e-169	89.5%
437	KR652009	Diplonevra nitidula voucher BIOUG02656-C05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	607.065	8.35e-169	89.5%
438	KR752065	Diplonevra nitidula voucher BIOUG01301-F11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	607.065	8.35e-169	89.5%
439	KR766434	Diplonevra nitidula voucher BIOUG16090-E03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	607.065	8.35e-169	89.5%
440	KT076735	Diplonevra nitidula voucher BIOUG11224-H06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	607.065	8.35e-169	89.5%
441	MG290322	Phorinae sp. BIOUG32614-B08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	607.065	8.35e-169	89.5%
442	KR740418	Diplonevra nitidula voucher BIOUG01301-A08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
443	KT701755	Phoridae sp. BOLD:AAU6538 voucher BIOUG22717-F10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
444	KT110932	Diplonevra nitidula voucher BIOUG01124-F02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
445	KR753044	Phoridae sp. BOLD-2016 voucher BIOUG01301-H08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
446	KR751172	Diplonevra nitidula voucher 10PHMAL-1197 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
447	OQ532333	Phoridae sp. voucher ZRCBDP0375408 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
448	KR757117	Diplonevra nitidula voucher BIOUG01301-C07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
449	KR643979	Diplonevra nitidula voucher BIOUG02622-D11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
450	KR714503	Megaselia sp. BOLD-2016 voucher BIOUG13245-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
451	MG103809	Megaselia sp. BIOUG24395-H09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
452	KR747600	Megaselia sp. BOLD-2016 voucher 10PHMAL-2791 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	526	92.3%	605.082	3.30e-168	89.5%
453	KM940128	Megaselia sp. BOLD:AAG3274 voucher BIOUG07045-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	609.047	2.11e-169	89.4%
454	KT077295	Megaselia rufipes voucher BIOUG10414-D08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
455	KR637255	Phoridae sp. BOLD-2016 voucher BIOUG02648-A02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
456	OR927035	Megaselia brevicostalis voucher SMNS_1198046 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
457	KP042056	Megaselia sp. BOLD:AAG3274 voucher BIOUG02745-C08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
458	KR762558	Megaselia rufipes voucher BIOUG13065-H10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
459	MG102150	Megaselia rufipes voucher BIOUG32321-B01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
460	KT075900	Megaselia rufipes voucher BIOUG10829-G08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
461	JF879879	Megaselia rufipes voucher BIOUG	530	93.0%	605.082	3.30e-168	89.4%
462	KP040787	Megaselia sp. BOLD:AAG3274 voucher BIOUG06927-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
463	KR757905	Megaselia rufipes voucher BIOUG01488-E09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
464	KM951269	Megaselia sp. BOLD:AAG3274 voucher BIOUG06117-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
465	KP046844	Megaselia sp. BOLD:AAG3274 voucher BIOUG04102-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
466	KR645957	Megaselia rufipes voucher BIOUG02596-H09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
467	KR634353	Megaselia rufipes voucher BIOUG02598-H10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
468	KM936873	Megaselia sp. BOLD:AAG3274 voucher BIOUG04255-D06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
469	KP047481	Megaselia sp. BOLD:AAG3274 voucher BIOUG06820-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
470	KR431321	Megaselia sp. BOLD:AAG3274 voucher BIOUG03577-F04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
471	KR509815	Phoridae sp. BOLD:AAG3274 voucher BIOUG18367-F10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
472	KM956146	Megaselia sp. BOLD:AAG3274 voucher BIOUG06899-B04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
473	OR927603	Megaselia brevicostalis voucher SMNS_1197907 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
474	KR719122	Megaselia rufipes voucher BIOUG13666-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
475	KT091738	Megaselia rufipes voucher BIOUG11057-A05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
476	KT108762	Megaselia rufipes voucher BIOUG02828-D08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
477	KT077147	Megaselia rufipes voucher BIOUG10528-G01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
478	KM917682	Megaselia sp. BOLD:AAG3274 voucher BIOUG09292-D12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
479	KR637265	Megaselia sp. BOLD-2016 voucher BIOUG02647-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
480	KT093051	Megaselia rufipes voucher BIOUG11319-B11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
481	KR642417	Megaselia rufipes voucher BIOUG02596-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
482	KT094959	Megaselia rufipes voucher BIOUG08823-D10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
483	KM946552	Megaselia sp. BOLD:AAG3274 voucher BIOUG07045-F12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
484	MG288800	Megaselia rufipes voucher BIOUG32542-C09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
485	OR930861	Megaselia perfusca voucher SMNS_1205572 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
486	KT112520	Megaselia rufipes voucher BIOUG02849-G05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
487	KP042007	Megaselia sp. BOLD:AAG3274 voucher BIOUG06996-H08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
488	KT101761	Megaselia rufipes voucher BIOUG02827-G03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
489	KM942990	Megaselia sp. BOLD:AAG3274 voucher BIOUG03481-B07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
490	KT086023	Megaselia rufipes voucher BIOUG10509-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
491	KT707129	Megaselia rufipes voucher BIOUG24009-G04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
492	KM969079	Megaselia sp. BOLD:AAG3274 voucher BIOUG05923-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
493	KR654953	Megaselia rufipes voucher BIOUG09927-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
494	KR751241	Megaselia rufipes voucher BIOUG09286-C05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
495	KR525238	Megaselia longicostalis voucher BIOUG14430-E12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
496	KM638582	Megaselia sp. BOLD:AAG3274 voucher BIOUG07039-F11 cytochrome oxidase subunit 1 (COI) gene, promoter region and partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
497	KP045808	Megaselia sp. BOLD:AAG3274 voucher BIOUG07024-B08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
498	KM932251	Megaselia sp. BOLD:AAG3274 voucher BIOUG07573-B01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	530	93.0%	605.082	3.30e-168	89.4%
499	OM314455	Phoridae sp. voucher BIOUG24772-F10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	535	93.9%	607.065	8.35e-169	89.3%
500	OM594213	Phoridae sp. voucher BIOUG23828-B02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	535	93.9%	607.065	8.35e-169	89.3%

Selected alignment ⓘ

Selected taxonomy ⓘ

Domain
Kingdom
Phylum
Class
Order
Family
Genus
Species

Analyst evaluation

Taxa of interest (TOI) analysis

Flag 2B

Outcome: None of the taxa of interest matched the candidates species.
Reasoning: Taxon of interest NOT detected in candidate species.

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage

Megaselia scalaris

Did not match any candidate

This Taxon of Interest has been independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of this taxon. Insufficient coverage can result in that taxon not be correctly identified as the taxonomic identity of the sample (type-II error).

Reference database: BLAST Core Nt

	Coverage of Megaselia scalaris
	Coverage of species in genus Megaselia
	Coverage of species in genus Megaselia in country of origin India

Database coverage of Megaselia scalaris

Flag 5.1A: The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)

185 records

There are 185 sequence records in the reference database for Megaselia scalaris that have been annotated with the given locus COI. It is possible that more records exist for this taxon which were not correctly annotated with this locus.

Global occurrence records for Megaselia scalaris.
Note that the occurrence data are not exhaustive, since the data fetched are limited to 5000 occurrence records. It is therefore possible for this species to occur in regions not shown on the map.

Database coverage of species in genus Megaselia

Flag 5.2B: The reference data offers some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus

/ (%) of species have sequence records in the reference database for:

Species in the genus Megaselia
At the target locus COI

Number of GenBank records at locus COI

Database coverage of species in genus Megaselia that occur in country of origin India

Flag 5.3B: The reference data offers some support for species in this genus, when we evaluate only species that occur in the sample's country of origin
Reasoning: Not all species in genus from the country of origin have reference sequence(s) for this locus

/ (%) of species have sequence records in the reference database for:

Species in the genus Megaselia
At the target locus COI
In the sample country of origin India

Number of GenBank records at locus COI

Publications supporting taxonomic association

This analysis evaluates how many independent publication events have contributed matching sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Candidates

Independent publication sources

Flag 4B

Outcome: Reference sequence sources lack diversity and may therefore be unreliable.
Reasoning: Matching sequence records for this species have only 1-5 independent sources.

(Found 1 sources)

1 Independent Source

The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession	Automated ⓘ	Authors	Title	Journal
KR666968	False	Hebert,P.D. Ratnasingham,S. Zakharov,E.V. Telfer,A.C. Levesque-Beaudin,V. Milton,M.A. Pedersen,S. Jannetta,P. deWaard,J.R.	Counting animal species with DNA barcodes: Canadian insects	Philos. Trans. R. Soc. Lond., B, Biol. Sci. 371 (1702) (2016) In press
KR666968	False	Hebert,P.D.N. Ratnasingham,S. Zakharov,E.V. Dewaard,J.R.	Direct Submission	Submitted (11-MAY-2015) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road EAST, Guelph, Ontario N1G2W1, Canada

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species" clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

Click + drag to pan

Scroll to zoom in/out

Click a label to view NCBI record

This phylogenetic tree shows the genetic relationship between the query sequence (labelled QUERY) and matching reference sequences (labelled species/accession). The tree was computed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with Phylocanvas.GL .

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa	Database
General	GBIF
General	ITIS
Mealybugs & scale	ScaleNet database
Thrips	Thripswiki
Spider Mites	Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice)	Psocodea Species File Online
Orthoptera	Orthoptera Species File Online
Drosophilidae	TaxoDros
Diptera	Catalog of the Diptera of the Australasian and Oceanian Regions Systema Dipterorum
Aphids	Aphid Species File
Ants	AntWeb AntCat
Lepidoptera (butterflies and moths)	The Global Lepidoptera Names Index
Gracillariidae (primitive moths)	Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths)	Global Information System on Pyraloidea
Tortricidae (tortrix moths)	Tortricidae Resources on the Net

`blast`	`2.16.0+`
`fastme`	`2.1.6.3`
`mafft`	`7.52`
`qcif/taxaplus`	`v1.0.0`
`Nextflow`	`24.10.6`

Flag	Analysis	Explanation	Outcome	Level
1A	Candidate selection	1 candidate species matched with high stringency (identity ≥ 98.5%)	Positive species identification	1
1B	Candidate selection	2-3 candidate species matched with high stringency (identity ≥ 98.5%)	The analyst should attempt subjective species identification at the genus level	2
1C	Candidate selection	>3 candidate species matched with high stringency (identity ≥ 98.5%)	The analyst should attempt subjective species identification at the genus level	3
1D	Candidate selection	At least one candidate species matched with moderate stringency (identity ≥93.5% and <98.5%)	The analyst should attempt subjective species identification at the genus level	2
1E	Candidate selection	No candidate species matched	Identification not possible (potential unknown species)	3
2A	Taxa of interest analysis	Taxon of interest detected in candidate species	At least one of the taxa of interest matched the candidates species	1
2B	Taxa of interest analysis	Taxon of interest NOT detected in candidate species	None of the taxa of interest matched the candidates species	3
2NA	Taxa of interest analysis	No Taxa of Interest were provided	Could not be assessed	0
4A	Supporting publications	Matching sequence records for this species are from >5 independent sources	Reference sequences are from diverse sources and therefore likely to be reliable	1
4B	Supporting publications	Matching sequence records for this species have only 1-5 independent sources	Reference sequence sources lack diversity and may therefore be unreliable	2
5NA	Database coverage	Criteria to perform this analysis were not met	Database coverage could not be assessed for this taxon.	0
5B	Database coverage	Locus was not provided for this sample	Database coverage could not be fully assessed for this taxon.	2
5.1A	Database representation for target taxon	The given locus for this taxon is well represented in reference database (>5 entries)	The reference data supports this taxon well	1
5.1B	Database representation for target taxon	The given locus for this taxon has limited representation in reference database (≤5 entries)	The reference data has some support for this taxon	2
5.1C	Database representation for target taxon	The given locus for this taxon is not present in reference database (0 entries)	The reference data are likely to be unreliable for this species	3
5.1NA	Database representation for target taxon	Assessment of related species is only possible for taxa at rank genus/species	This taxon could not be fully assessed	0
5.2A	Database representation for related species	>90% of related taxa have reference sequence(s) at the given locus	The reference data supports species in this genus well	1
5.2B	Database representation for related species	10-90% of related taxa have reference sequence(s) at the given locus	The reference data offers some support for species in this genus	2
5.2C	Database representation for related species	≤10% of taxon have reference sequence(s) at the given locus	The reference data offers little support for species in this genus	3
5.2NA	Database representation for related species	Assessment of related species is only possible for taxa at rank genus/species	This taxon could not be fully assessed	0
5.3A	Database representation for related species in country of origin	All species in genus from country of origin have reference sequence(s) for this locus	The reference data supports species in this genus well, when we evaluate only species that occur in the sample's country of origin	1
5.3B	Database representation for related species in country of origin	Not all species in genus from the country of origin have reference sequence(s) for this locus	The reference data offers some support for species in this genus, when we evaluate only species that occur in the sample's country of origin	2
5.3C	Database representation for related species in country of origin	No species in genus have been observed in the country of origin	It's unlikely that the true taxonomic identity is another species in this genus, because no others have been recorded in the sample's country of origin	0
5.3NA	Database representation for related species in country of origin	Assessment of related species is only possible for taxa at rank genus/species	This taxon could not be fully assessed	0
6A	Phylogenetic assessment	Candidate species grouped within single clade	The query sequence is genetically consistent with sequences of the best-matching species	1
6B	Phylogenetic assessment	The query sequence clusters as sister to a species-specific clade or as a single branch in a tree	The sample is genetically distinct from the sequences of the best-matching species, and may lack representation in the reference database. This indicates that the sample organism is either poorly studied or a potential novel species.	2
6C	Phylogenetic assessment	Genotype diversity grouped into multiple diverse groups	The top candidate species cannot be confidently distinguished from other related species	3
7A	Preliminary identification confirmation	Identified species is consistent with preliminary ID	The preliminary identification is supported by the molecular data	1
7B	Preliminary identification confirmation	Identified species is not consistent with preliminary ID	The molecular data contradicts the preliminary identification	3
7NA	Preliminary identification confirmation	Insufficient evidence due to inconclusive taxonomy of the sample	Cannot confirm or reject the preliminary ID	0

Taxonomic identification report

Result overview

Sample metadata ⓘ

Input DNA sequence

Workflow parameters

Workflow program versions

Analyst evaluation

Identification of candidate species

Candidate species

BLAST search results

Analyst evaluation

Taxa of interest (TOI) analysis

Publications supporting taxonomic association

Analyst evaluation

Intraspecies diversity

Analyst evaluation

Taxonomy Check