Taxonomic identification report

Sample ID: LC438549.1
Reference database: BLAST

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Report generated from the Taxonomic Identification Nextflow workflow. The analysis is fully documented here.

Facility Cairns Airport
Analyst John Doe
Analysis start 2025-08-17 15:18:16
Analysis end 2025-08-22 19:36:56
Wall time 124:18:40 hours

This report describes the results of a taxonomic identification analysis for the sample LC438549.1. The analysis compares the sample's DNA sequence against a reference database of sequences with known taxonomy. The results include the best matches from the database, which are referred to as "Candidates". To provide rigour, the report also includes an analysis of supporting publications (a measure of the integrity of candidate hit sequences), and database coverage of the different taxa that are of interest to the report.

Sample metadata

Sample ID LC438549.1
Locus COI
Preliminary ID Asterias rubens
Taxa of interest Asteridae
Acanthaster planci
Country Japan
Host driftwood
Sequencing platform nanopore
Query DNA sequence View

Anneissia japonica

Outcome: Positive species identification, pending verification of reference data.
Reasoning: 1 candidate species matched with high stringency (identity ≥ 98.5%), but reference sequences lack independent publications.
Matching species: Strong: 1 Moderate: 4 Weak: 82
Candidate species
Candidate Species Hits Sources Max Identity Min Identity Genus Coverage
Anneissia japonica 3 3 100.0% 100.0% 56%
Preliminary ID
Species Confirmed? DB records Genus coverage Coverage report
Asterias rubens
143 16%
Outcome: The molecular data contradicts the preliminary identification.
Reasoning: Identified species is not consistent with preliminary ID.
Taxa of Interest
Taxon Detected? DB Records Genus Coverage Coverage report
Asteridae
- -
Acanthaster planci
524 75%
Outcome: None of the taxa of interest matched the candidates species.
Reasoning: Taxon of interest NOT detected in candidate species.

Analyst evaluation

Identification of candidate species

Outcome: Positive species identification.
Reasoning: 1 candidate species matched with high stringency (identity ≥ 98.5%).

BLAST hits must meet ONE of these criteria to be considered for candidate screening:

Minimum alignment length 300bp
Minimum query coverage 85.0%

BLAST hits have been classified as follows:

Classification Alignment identity Number of hits Number of species
STRONG MATCH ≥ 98.5% 3 1
MODERATE MATCH ≥ 93.5% 17 4
NO MATCH < 93.5% 480 82

Lowest identity of all 500 BLAST hits: 87.5%

Candidate species

Species No. hits Top identity Median identity Min identity Top E-value Coverage Publications
Anneissia japonica 3 100.0% 100.0% 100.0% 0.0

Analyst evaluation

Taxa of interest (TOI) analysis

Outcome: None of the taxa of interest matched the candidates species.
Reasoning: Taxon of interest NOT detected in candidate species.

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage
Asteridae Did not match any candidate
Acanthaster planci Did not match any candidate

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa Database
General GBIF
General ITIS
Mealybugs & scale ScaleNet database
Thrips Thripswiki
Spider Mites Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice) Psocodea Species File Online
Orthoptera Orthoptera Species File Online
Drosophilidae TaxoDros
Diptera Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum
Aphids Aphid Species File
Ants AntWeb
AntCat
Lepidoptera (butterflies and moths) The Global Lepidoptera Names Index
Gracillariidae (primitive moths) Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths) Global Information System on Pyraloidea
Tortricidae (tortrix moths) Tortricidae Resources on the Net