Taxonomic identification report

Sample ID: ON075825
Reference database: BLAST

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Report generated from the Taxonomic Identification Nextflow workflow

This report describes the results of a taxonomic identification analysis for the sample ON075825. The analysis compares the sample's DNA sequence against a reference database of sequences with known taxonomy. The results include the best matches from the database, which are referred to as "Candidates". To provide rigour, the report also includes an analysis of supporting publications (a measure of the integrity of candidate hit sequences), and database coverage of the different taxa that are of interest to the report.

Facility Cairns Airport
Analyst John Doe
Analysis start 2025-05-10 07:00:00
Analysis end 2025-05-29 14:52:17
Wall time 463:52:17 hours

Result overview

Inconclusive

Outcome: The analyst should attempt subjective species identification at the genus level.
Reasoning: 2-3 candidate species matched with high stringency (identity ≥ 98.5%).
Preliminary morphology ID Epinephelus confirmed? NA
Outcome: Insufficient evidence due to inconclusive taxonomy of the sample.
Reasoning: Cannot confirm or reject the preliminary ID.
Taxa of interest detected? True
Outcome: At least one of the taxa of interest matched the candidates species.
Reasoning: Taxon of interest detected in candidate species.
Outcome: The reference data supports this taxon well.
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries).
Outcome: The reference data offers some support for species in this genus.
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus.

Sample metadata

Locus COX1
Preliminary ID Epinephelus
Taxa of interest Epinephelus
Epinephelus awoara
Country China
Host fishing net
Sample ID ON075825
Locus provided True
Query DNA sequence

Analyst evaluation

Identification of candidate species

Outcome: The analyst should attempt subjective species identification at the genus level.
Reasoning: 2-3 candidate species matched with high stringency (identity ≥ 98.5%).

BLAST hits must meet ONE of these criteria to be considered for candidate screening:

Minimum alignment length 300bp
Minimum query coverage 85.0%

BLAST hits have been classified as follows:

Classification Alignment identity Number of hits Number of species
STRONG MATCH ≥ 98.5% 3 3
MODERATE MATCH ≥ 93.5% NA NA
NO MATCH < 93.5%

Candidate species

Species Hits Identity E-value Database coverage
Epinephelus coioides 1 100.0% 0.0
Epinephelus lanceolatus 1 100.0% 0.0
Epinephelus moara 1 98.7% 0.0

Analyst evaluation

Taxa of interest (TOI) analysis

Outcome: At least one of the taxa of interest matched the candidates species.
Reasoning: Taxon of interest detected in candidate species.

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage
Epinephelus genus Epinephelus Epinephelus coioides ON075825 1.0
Epinephelus awoara Did not match any candidate

Publications supporting taxonomic association

This analysis evaluates how many independent publication events have contributed matching sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Independent publication sources

Outcome: Reference sequence sources lack diversity and may therefore be unreliable.
Reasoning: Matching sequence records for this species have only 1-5 independent sources.

(Found 1 sources)

1 Independent Source

The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
ON075825 False Zheng,Q.
Guo,X.
Wang,W.
Huang,Y.
Huang,X.
Qin,Q.
Direct Submission Submitted (24-MAR-2022) College of Marine Sciences, South China Agricultural University, No.483 Wushan Road, Guangzhou, Guangdong 510642, China
ON075825 False Zheng,Q.
Guo,X.
Wang,W.
Huang,Y.
Huang,X.
Qin,Q.
Direct Submission Submitted (24-MAR-2022) College of Marine Sciences, South China Agricultural University, No.483 Wushan Road, Guangzhou, Guangdong 510642, China
ON075825 False Zheng,Q.
Guo,X.
Wang,W.
Huang,Y.
Huang,X.
Qin,Q.
Direct Submission Submitted (24-MAR-2022) College of Marine Sciences, South China Agricultural University, No.483 Wushan Road, Guangzhou, Guangdong 510642, China
ON075825 False Zheng,Q.
Guo,X.
Wang,W.
Huang,Y.
Huang,X.
Qin,Q.
Direct Submission Submitted (24-MAR-2022) College of Marine Sciences, South China Agricultural University, No.483 Wushan Road, Guangzhou, Guangdong 510642, China
ON075825 False Zheng,Q.
Guo,X.
Wang,W.
Huang,Y.
Huang,X.
Qin,Q.
Direct Submission Submitted (24-MAR-2022) College of Marine Sciences, South China Agricultural University, No.483 Wushan Road, Guangzhou, Guangdong 510642, China
Show Hide 3 more publications...

Independent publication sources

Outcome: Reference sequence sources lack diversity and may therefore be unreliable.
Reasoning: Matching sequence records for this species have only 1-5 independent sources.

(Found 1 sources)

1 Independent Source

The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
XM_033618714 True No publications for this record

Independent publication sources

Outcome: Reference sequence sources lack diversity and may therefore be unreliable.
Reasoning: Matching sequence records for this species have only 1-5 independent sources.

(Found 1 sources)

1 Independent Source

The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
XM_050051969 True No publications for this record

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species" clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

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Click a label to view NCBI record

This phylogenetic tree shows the genetic relationship between the query sequence (labelled QUERY) and matching reference sequences (labelled species/accession). The tree was computed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with Phylocanvas.GL .

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa Database
General GBIF
General ITIS
Mealybugs & scale ScaleNet database
Thrips Thripswiki
Spider Mites Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice) Psocodea Species File Online
Orthoptera Orthoptera Species File Online
Drosophilidae TaxoDros
Diptera Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum
Aphids Aphid Species File
Ants AntWeb
AntCat
Lepidoptera (butterflies and moths) The Global Lepidoptera Names Index
Gracillariidae (primitive moths) Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths) Global Information System on Pyraloidea
Tortricidae (tortrix moths) Tortricidae Resources on the Net